DNA Probes

MeSH ID: D015342

DEFINITION: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.

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administration & dosage [Q000008] ; adverse effects [Q000009] ; agonists [Q000819] ; analysis [Q000032] ; antagonists & inhibitors [Q000037] ; biosynthesis [Q000096] ; blood [Q000097] ; cerebrospinal fluid [Q000134] ; chemical synthesis [Q000138] ; chemistry [Q000737] ; classification [Q000145] ; contraindications [Q000744] ; diagnostic use [Q000176] ; drug effects [Q000187] ; economics [Q000191] ; genetics [Q000235] ; history [Q000266] ; immunology [Q000276] ; isolation & purification [Q000302] ; metabolism [Q000378] ; pharmacokinetics [Q000493] ; pharmacology [Q000494] ; physiology [Q000502] ; poisoning [Q000506] ; radiation effects [Q000528] ; secretion [Q000557] ; standards [Q000592] ; supply & distribution [Q000600] ; therapeutic use [Q000627] ; toxicity [Q000633] ; ultrastructure [Q000648] ; urine [Q000652]

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